ASTRA Clubs in District Three
| Altrusa Sponsoring Club | ASTRA Club | Year Chartered |
| Anderson, SC | T.L. Hanna HS ASTRA Club | 1968 |
| Caldwell County, NC | ASTRA of West Caldwell County HS | 2007 |
| Gainesville, FL | University of Florida ASTRA | |
| Ocala, FL | Forest High School ASTRA | 1989 |
| Ocala, FL | Lake Weir HS ASTRA | In progress |
| Spartanburg, SC | Dorman HS ASTRA | 1967 |
| Spartanburg, SC | Spartanburg, SC | 1995 |